Urokinase (UK) is a serine protease which is one of
biological plasminogen activators. It is involved
in a number of biological functions including fibrinolysis,
embryogenesis, cell migration, tissue remodeling, ovulation, and wound
healing. It can be obtained from human urine or kidney cell culture. UK is a
two chain glycoprotein containing 411 amino acids with 12 dissulfide
bonds. Its molecular weight is 54,000.
Greater than 90% high molecular weight UK by SDS-PAGE.
Greater than 100,000 IU/mg.
1 nM UK will cause a change in absorbance of 0.001
at 405 nm in 1 minute at room temperature in 100 ul
0.05 M Tris-HCl, 0.1 M NaCl,
pH 7.4, using S2444 (0.6 mM) as the substrate.
White lyophilized powder or solution.
The lyophilized powder, though stable at room temperature, is best stored
desiccated below 0°C. It is soluble in water or other buffered solution.