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GLYCOSYLATED RECOMBINANT HUMAN PROUROKINASE


Other Product Description
VEGF-165
Aprotinin
Urokinase
Prourokinase Non-glycosylated
Prourokinase Glycosylated
ATF
ATF II
Tissue Plasminogen
Activator

Glu-plasminogen
Lys-plasminogen
Plasmin
ETI

rEK

K2tPA

 

 

DESCRIPTION:
Prourokinase (Pro-UK) is the precursor of two chain urokinase (UK), a serine protease that can activate plasminogen to plasmin. Pro-UK can be converted into UK via hydrolysis of the Lys-158-Ile-159 peptide bond by certain proteases such as plasmin, kallikrein or cathepsin B. Although Pro-UK is a zymogen and is inert in plasma, it has a higher intrinsic activity than most other zymogen. In the presence of fibrin fragment E, plasminogen activation by the intrinsic activity of Pro-UK is promoted 500-fold, giving it an activity comparable to UK. Pro-UK is a fibrin specific thrombolytic agent consisting of 411 amino acids with 12 disulfide bonds. Its molecular weight is 54,000.

SOURCE:
CHO cells

PURITY:
Greater than 90% by SDS-PAGE.

SPECIFIC ACTIVITY:
After activation by plasmin, its specific activity is greater than 100,000 IU/mg. Without activation it only has less than 1% of activity.

ACTIVITY:
After activation by plasmin, 1 nM UK will cause a change in absorbance of 0.001 at 405 nm in 1 minute at room temperature in 100 ul 0.05 M Tris-HCl, 0.1 M NaCl, pH 7.4, using S2444 (0.6 mM) as the substrate.

PRESENTATION:
White lyophilized powder or solution.

STORAGE:
The lyophilized powder is best stored desiccated below 0C. After it is reconstituted with sterile water or other buffered solutions (we recommend to dissolve pro-UK with 5mM HAc-NaAc, pH 4.8), it should be stored at -20C.


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