Plasminogen is a single chain glycoprotein zymogen which is synthesized in the liver and circulates
in plasma at a concentration of approximately 2.0 uM.
Plasminogen can be activated to plasmin
via t-PA, u-PA or streptokinase, resulting in hydrolysis of the
Arg-560-Val-561 peptide bond, yielding two chains which remain covalently
associated by a dissulfide bond. Glu-plasminogen is a monomer containing 790 amino acids
with 24 dissulfide bonds, no free sulfhydryl groups, an N-terminal glutamic
acid, and a C-terminal asparagine. The molecular
weight is 92,000.
Human plasma that has been shown by certified tests to be negative for HBsAg and for HIV antibodies.
Single band by SDS-PAGE. Plasmin activity is not
detectable. Glu-plasminogen form is verified by
acid-urea gel electrophoresis.
After activation by urokinase, 1 nM plasmin will cause a change
in absorbance of 0.001 at 405 nm in 1 minute at room temperature in 100ul
0.05M Tris-HCl, 0.1 M NaCl,
pH 7.4, using S2251(1.5 mM) as the substrate.
Solution in 0.05M Tris-HCl, 0.1M NaCl, pH 7.4
It should be stored at –20 °C. Avoid freeze/thaw cycles.