Recombinant Enterokinase (rEK)
is the catalytic subunit of bovine enterokinase
with a Cys112->Ala mutation.
rEK is expressed and isolated from the yeast Saccharomyces Cerevisin to yield a highly purified enzyme
preparation. rEK recognizes the identical cleavage site as the native enzyme (i.e.
AspAspAspAspLys). rEK can be used to cleave any
fusion protein containing the recognition sequence. With the free Cys112
mutated to Ala, rEK may have a better stability. The molecular weight of rEK is about 38kd due to glycosylation.
95% by SDS-PAGE.
One unit is defined as the amount of enzyme needed to cleave 50 mg of
fusion protien 16 hours at 25°C in 50 mM Tris-HCl, 50 mM NaCl, 2mM CaCl2, pH7.5.
Solution in 50 mM Tris-HCl,
0.2M NaCl, 20% Glycerol, pH7.0.
Store at -20°C.