Plasminogen is a single chain glycoprotein zymogen which is synthesized in the liver and circulates
in plasma at a concentration of approximately 2.0 uM.
Plasminogen can be activated to plasmin
via t-PA, u-PA or streptokinase, resulting in hydrolysis of the
Arg-560-Val-561 peptide bond, yielding two chains which remain covalently
associated by a dissulfide bond. Lys-plasminogen is purified from Glu-plasminogen
after restricted digestion by plasmin. The reaction
results in the release of a 76 residue peptide (Glu-1-Lys-76). The molecular
weight of Lys-plasminogen is 83,000.
Human plasma that has been shown by certified tests to be negative for HBsAg and for HIV antibodies.
Single band by SDS-PAGE. Plasmin activity is not
detectable. Lys-plasminogen is verified by
acid-urea gel electrophoresis.
After activation by urokinase, 1 nM plasmin will cause a change
in absorbance of 0.001 at 405 nm in 1 minute at room temperature in 100 ul 0.05 M Tris-HCl, 0.1 M NaCl, pH 7.4, using S2251 (1.5 mM)
as the substrate.
Solution in 0.05 M Tris-HCl, 0.1M NaCl, pH 7.4
It should be stored at -20 °C. Avoid freeze/thaw cycles.