Reteplase, or K2tPA, is a non-glycosylated deletion mutant variant of human tissue plasminongen activator (tPA)
produced by E.coli using recombinant technology. Reteplase is made up of 355 amino acids, including amino
acids 1-3 and 176-527 of native tPA. Reteplase consists of the kringle2 and the protease
domain of tPA and has a moderate affinity for
fibrin. In the presence of fibrin, plasminogen
activation by Reteplase can be promoted. The
molecular weight of Reteplase is 39571 daltons.
Greater than 90% by SDS-PAGE.
After conversion to two-chain form completely, 1 nM Reteplase will cause a
change in absorbance of 0.001 at 405 nm in 1 minute at room temperature in
100 ml 50mM Tris-HCl, 100mM NaCl,
pH 7.4, using S2288 (0.6 mM) as the substrate.
White lyophilized powder or solution.
The lyophilized powder is best stored desiccated below 0°C.