LANDING BIOTECH INC
Polyclonal Antibody Production
Peptide Synthesis and Purification
- Expression plasmid construction: the gene of interest will be amplified and isolated from the customer-supplied vector and subcloned into a prokaryotic expression vector. The authenticity of the target gene will be confirmed by PCR, restriction enzyme digest and DNA sequencing.
- Transformation: transform expression plasmid into a high-efficiency expression bacterial host.
- Small-scale induction and expression: the expression of target proteion is tested in 3ml culture medium at various of temperatures. The expression rate and the size of target protein will be evaluated by SDS-PAGE.
- Large-scale induction and expression: Certain amount of bacterial culture (depending on the quantity of target protein) will be induced and harvest for protein purification.
- Inclusion body isolation and renaturation*: Purify the inclusion body of target protein, recover its activity by in vitro refolding.
- Purification: target protein will be purified at either native or denature conditions with affinity chromatography (i.e. Ni-NTA for proteins with 6XHis tag, glutathione-beads for proteins with GST-tag, chitin-beads for proteins with CBD-tag).
*: this step will be omitted if the target protein is soluble and active in bacterial lysate.
| Timeround: | 2-4 weeks |
| Presentation: | solution or lyophilized powder |
| Storage: | -20°C |
Custom Protein Expression and Purification Catalog
| Catalog Number | Size | Purity | Price |
| PEP-1A | 1-5mg | >75% | $2400 |
| PEP-1B | 1-5mg | >90% | $2800 |
| PEP-2A | 10-25mg | >75% | $2750 |
| PEP-2B | 10-25mg | >90% | $3200 |
| PEP-3A | 1-1000g | >75% | Inquire |
| PEP-3B | 1-1000g | >90% | Inquire |